Human IL-12/IL-23p40 Precoated ELISA Kit
$499.00
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Key Features
✓ Assay Range: 31.3-2000 pg/mL
✓ Sensitivity: 15 pg/mL
✓ Sample Types: Serum, Plasma, Cell Culture Supernates
Size
✓ 1 Kit (96 well plate)
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Product Type | Quantitative ELISA, Colorimetric |
| Product Summary |
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| Reactivity | Human IL-12/IL-23p40
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| Product Components |
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Storage & Preparation
| Shipping | Shipped on blue ice |
| Stability & Storage | Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration date. |
| Reconstitution | See Datasheet for Component Preparation |
Data Images
Human IL-12/IL-23p40 ELISA standard curve, typical data. User-generated standard curve is required for each set of samples. Please refer to protocol.
Background
| Alternative Names | Interleukin-12, IL12, p70, p40, Natural Killer Cell Stimulatory Factor, NKSF, cytotoxic lymphocyte maturation factor, CLMF |
| Function | Human IL-12p40 is a multifunctional subunit as a component of the heterodimeric cytokines IL-12 and IL-23. IL-12p40 pairs with the p35 subunit to form IL-12 and with the p19 subunit to form IL-23. Both cytokines play critical roles in shaping immune responses. IL-12 is pivotal for Th1 differentiation and IFN-gamma production, while IL-23 is involved in the expansion and maintenance of Th17 cells. Beyond its role in heterodimer formation, IL-12p40 can exist as a monomer or form homodimers. The p40 homodimer has been shown to act as a natural antagonist by binding to the IL-12 receptor (specifically the IL-12R beta1 subunit) without initiating the full signaling cascade, thereby modulating the activity of IL-12 and IL-23. |
| Tissue Specificity | IL-12p40 is primarily expressed by antigen-presenting cells such as dendritic cells, macrophages, and monocytes, with additional contributions from activated B cells and, under certain conditions, other cell types like microglia or epithelial cells. It is notably produced in secondary lymphoid organs (e.g., lymph nodes and spleen) and in peripheral tissues, particularly during inflammatory responses. |
| Cellular Localization | Secreted |
| Involvement in Disease | Since IL-12p40 is a shared subunit for both IL-12 and IL-23, its imbalance can impact several downstream immune pathways. In conditions such as Crohn’s disease and ulcerative colitis, dysregulated IL-12p40 expression is implicated in intestinal inflammation. Its role in driving Th1 and Th17 responses (via IL-12 and IL-23, respectively) can exacerbate mucosal immune responses. The IL-12/IL-23 pathway is central to the pathogenesis of psoriasis, participating in the inflammatory milieu that drives the proliferation of keratinocytes and the development of psoriatic plaques. |
| UniProt | P29460 |
| Gene Symbol | IL12A |
| Entrez Gene ID | 3592 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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