July 1, 2024

ELISAs

Sandwich-based enzyme-linked immunoassays centered around matched antibody pairs tested for specificity and reproducibility.

Complete ELISA Kits to Get Results Quickly and Accurately

Sandwich-based enzyme-linked immunoassays centered around tested matched antibody pairs for specificity and reproducibility. Our ELISA kits contain precoated plates and all the reagents required to perform an ELISA. Our ELISA kits are suitable for serum, plasma and cell culture supernates.

✓ Quick assay time. Less than 3 hours. Optimized protocol for accurate results.

✓ Specificity for target protein. Selected matched antibody pairs.

✓ High reproducibility. Low % CVs.

✓ Sensitivity in low pg/mL.

ELISA Kits for Your Research Area

We offer ELISA kits for human, mouse and rat samples from a wide range of cytokine and growth factor families.

  • Interleukins: IL-1 beta, IL-2, IL-4, IL-6, IL-10 and more
  • Interferons: IFN-alpha and IFN-gamma
  • Growth Factors: EGF, HGF, TGF-beta 1, FGF-basic, VEGF and more
  • Chemokines: MCP-1/CCL2, IP-10/CXCL10, TARC/CCL17 and more
  • Additional Biomarkers: Adiponectin, C-Reactive Protein (CRP), Granzyme B, Resistin, TNF-alpha and more

ELISpot Kits for Single-Cell Detection

ELISpot Kits are widely used to detect analyte-secreting cells at the single-cell level. This gives ELISpots assays a unique advantage in measuring the antigen-specific immune response of immune cells and subtypes. ELISpots remain an important tool in vaccine research in terms of detecting T cell response.

✓ Ready-to-use kit.

✓ Kit includes: precoated PVDF plate, detection antibody, Streptavidin-HRP, AEC color development system, dilution buffer, wash buffer and cell stimulation reagent.

✓ You add your cells and design the experimental cell culture conditions in the ELISpot wells.

✓ Analyte-secreting cells will be detected by precipitation of captured analyte on the PVDF membrane. These “spots” are quantitated to determine the presence of individual analyte-secreting cells.

ELISpot Kits for Your Research Area

We offer ELISpot kits for human and mouse cell detection for the following cytokines:

✓ Interleukins: IL-2, IL-4, IL-10 and IL-17A

✓ Interferons: IFN-gamma

TNF-alpha

Example of IFN-gamma ELISpot detection of following stimulation with PMA and Ionomycin compared to no stimuli negative control (media only)

Side-by-side comparison of 2 ELISpot wells showing 533 spots indicating IFN-gamma secreting cells following PMA and Ionomycin treatment on the left, versus the untreated negative control on the right showing no spots (i.e., no IFN-gamma secreting cells)
Share on:
Facebook
Pinterest
WhatsApp
RELATED PRODUCTS
Featured Categories