EXact-Cut™ PspGI (EcoRII) Restriction Endonuclease
$49.00
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Key Features
✓ Reduced star activity
✓ Save time. Digest DNA in 5-15 minutes
✓ All Exact-Cut™ Enzymes 100% active in a single buffer (included)
✓ Purple Loading Dye included
Size
✓ 2,000 Units
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Product Details
Storage & Preparation
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Product Details
| Restriction Enzyme Site | 5ʹ…↓CCWGG…3ʹ 3ʹ…↑GGWCC…5ʹ Isoschizomers: EcoRI, MvaI, BstNI, BciT130I, BseBI, AjnI, Psp6I, Bst2UI (Isoschizomers may have different methylation sensitivities) |
| Unit Definition | One unit is defined as the amount of enzyme required to digest 1 ug of λ DNA in 1 hour at 37°C in a total reaction volume of 50 uL. |
| Recommended Reaction Conditions | 1 x EXact-Cut™ Buffer Incubate at 75°C Refer to datasheet for reaction setup |
| Heat Inactivation | 1. The enzyme cannot be inactivated at high temperature, and can be extracted using phenol chloroform extraction or column purification 2. Add appropriate volume of 6X Gel Loading Dye, according to the reaction system |
| Product Components | EXact-Cut™ PspGI (10 Units/uL): 2,000 Units EXact-Cut™ 10X Buffer: 2 x 1 mL 6X Gel Loading Dye, Purple: 2 x 1 mL |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | Store at -20°C for up to 24 months. |
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| Product Summary | EXact-Cut™ PspGI (EcoRII) Restriction Endonuclease is engineered for high specificity, reduced star activity, and time saving DNA digestion in 5-15 minutes. To simplify experimental design using multiple restriction enzymes, our entire range of EXact-Cut™ restriction endonucleases are 100% active in our EXact-Cut™ buffer (included) and are optimized for single-tube reactions along with digestion and ligation protocols. |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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