Product Type	Quantitative ELISA, Colorimetric
Product Summary	Assay Range: 3.9-250 pg/mL Sensitivity: 3 pg/mL Sample Types: Serum, Plasma, Cell Culture Supernates Intra-Assay Precision: ≤10% Inter-Assay Precision: ≤15%
Reactivity	Human EGF No cross-reactivity with human cytokines
Product Components	Human EGF Coated Microplate, 96 well (8 x12 strips) Human EGF Standard, 2 vials Human EGF Biotinylated Antibody, 2 vials Streptavidin-HRP, 2 vials Dilution Buffer, 3 bottles Washing Buffer, 1 bottle Color Reagent (TMB), 1 bottle Stop Solution, 1 bottle Plate Sealers, 4 strips Other materials required: See Datasheet

Shipping	Shipped on blue ice
Stability & Storage	Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration date.
Reconstitution	See Datasheet for Component Preparation

Alternative Names	Epidermal Growth Factor, Beta-Urogastrone, Urogastrone, URG, HOMG4
Function	EGF (Epidermal Growth Factor) is a growth factor that plays several roles related to cell growth, proliferation, and differentiation mediated through binding to its receptor, EGFR (Epidermal Growth Factor Receptor). EGF may be expressed in macrophages, platelets, plasma, milk, urine, saliva, seminal fluid, amniotic fluid and cerebrospinal fluid. Several roles are attributed to EGF such as angiogenesis, epithelial cell growth and maintenance, keratinocyte proliferation, gastrointestinal mucosa integrity, along with neurogenesis and neuronal survival. Due to these wide-ranging functions as a growth factor, EGF is commonly used in cell culture for the maintenance and growth of cell lines, stem cells and 3-D organoids.
Tissue Specificity	EGF expression has been reported in skin, gastrointestinal epithelial tissue, salivary glands, kidney, brain, liver, mammary glands, ovaries and testes.
Cellular Localization	Membrane-bound and cleaved soluble protein forms.
Involvement in Disease	Dysregulation of EGF is associated with several cancers (lung cancer, breast cancer, colorectal cancer, and head and neck cancer), psoriasis, inflammatory bowel disease (IBD), diabetic nephropathy, polycystic kidney disease, peptic ulcers and impaired wound healing.
UniProt	P01133
Gene Symbol	EGF
Entrez Gene ID	1950

Human EGF Precoated ELISA Kit

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