Human IL-12p70 Precoated ELISA Kit
$499.00
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Key Features
✓ Assay Range: 3.13-200 pg/mL
✓ Sensitivity: 3 pg/mL
✓ Sample Types: Serum, Plasma, Cell Culture Supernates
Size
✓ 1 Kit (96 well plate)
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Product Type | Quantitative ELISA, Colorimetric |
| Product Summary |
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| Reactivity | Human IL-12p70
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| Product Components |
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Storage & Preparation
| Shipping | Shipped on blue ice |
| Stability & Storage | Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration date. |
| Reconstitution | See Datasheet for Component Preparation |
Data Images
Human IL-12p70 ELISA standard curve, typical data. User-generated standard curve is required for each set of samples. Please refer to protocol.
Background
| Alternative Names | Interleukin-12, IL12, p70, p40, Natural Killer Cell Stimulatory Factor, NKSF, cytotoxic lymphocyte maturation factor, CLMF |
| Function | Interleukin-12 (IL-12) is a cytokine bridging innate and adaptive immunity, exerting effects on immune responses, particularly in the context of intracellular pathogens and tumor surveillance. IL-12 is instrumental in driving the differentiation of naïve CD4⁺ T cells into T helper type 1 (Th1) cells. IL-12 further stimulates these cells, as well as natural killer (NK) cells, to produce interferon-gamma (IFN-gamma). Additionally, IL-12 supports the activation and proliferation of cytotoxic T lymphocytes (CTLs). IL-12 is a disulfide-linked heterodimer composed of a 40 kD cytokine subunit encoded by the IL12B gene, and a 35 kD subunit encoded by the IL12A gene. The IL-12 receptor is a heterodimer composed of two subunits: IL-12R beta1 and IL-12R beta2. These subunits are expressed on the surface of T cells and NK cells, where the JAK/STAT signaling pathway—especially STAT4 activation—translates extracellular signals into effective immune responses. |
| Tissue Specificity | Human IL-12 is chiefly expressed by antigen-presenting cells (APCs)—especially dendritic cells and macrophages—with B cells contributing under specific conditions. These cells are primarily located in secondary lymphoid organs (lymph nodes and spleen) contributing to activities to prime adaptive immune responses but may be found in peripheral tissues where local immune reactions occur (skin, lungs, gastrointestinal tract). |
| Cellular Localization | Secreted |
| Involvement in Disease | Dysregulation of IL-12 is implicated in autoimmune and inflammatory diseases (rheumatoid arthritis, inflammatory bowel disease, psoriasis, multiple sclerosis), chronic infection, cancer (tumor microenvironment) and Graft-versus-Host Disease (GVHD). |
| UniProt | P29460 |
| Gene Symbol | IL12B |
| Entrez Gene ID | 3593 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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