Human sIL-2R Precoated ELISA Kit
$499.00
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Key Features
✓ Assay Range: 34.38-2200 pg/mL
✓ Sensitivity: 30 pg/mL
✓ Sample Types: Serum, Plasma, Cell Culture Supernates
Size
✓ 1 Kit (96 well plate)
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Product Type | Quantitative ELISA, Colorimetric |
| Product Summary |
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| Reactivity | Human sIL-2R
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| Product Components |
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Storage & Preparation
| Shipping | Shipped on blue ice |
| Stability & Storage | Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration date. |
| Reconstitution | See Datasheet for Component Preparation |
Data Images
Human sIL-2R ELISA standard curve, typical data. User-generated standard curve is required for each set of samples. Please refer to protocol.
Background
| Alternative Names | Interleukin-2 receptor subunit alpha, CD25, p55, IDDM10, IMD41, TCGFR |
| Function | The IL-2 receptor exists in different affinity forms, which are determined by the combination of its subunits. The IL-2Rα (CD25) subunit binds IL-2 with low affinity and does not transduce signals by itself. The IL-2Rβ (CD122) subunit is essential for signal transduction and contributes to the formation of an intermediate-affinity receptor when combined with the common γ chain (CD132) subunit. The full high-affinity IL-2 receptor is a heterotrimer composed of IL-2Rα, IL-2Rβ, and the common γ chain. In this configuration, the receptor is capable of binding IL-2 at very low concentrations, ensuring robust signaling during immune activation via JAK1, JAK3 and STAT5 pathways, as well as the PI3K/Akt pathway and MAPK pathway affecting various functions in T cell proliferation and differentiation.
Soluble IL-2R alpha (sIL-2Rα) is generated either by proteolytic cleavage (shedding) of the extracellular domain of the membrane-bound IL-2Rα or by alternative mRNA splicing. By binding IL-2 in the extracellular space, it can regulate the availability of the cytokine for binding to the high-affinity membrane-bound IL-2 receptor complex on T cells and other immune cells. This sequestration may limit IL-2-driven activation and proliferation under certain conditions. |
| Tissue Specificity | Expression of IL-2R alpha (CD25) is transiently upregulated on conventional CD4⁺ and CD8⁺ T cells following antigen stimulation, enabling these cells to form a high-affinity receptor for IL-2. CD25 is constitutively expressed at high levels on Tregs (CD4⁺ CD25⁺ FoxP3⁺ ). Although less prominent than in T cells, activated B cells can also express IL-2R alpha, particularly during immune responses, where IL-2 signaling may influence their function. A subset of NK cells upregulates IL-2R alpha upon activation, contributing to their responsiveness to IL-2. Some antigen-presenting cells (APCs), including dendritic cells and monocytes, can express IL-2R alpha in response to inflammatory signals, potentially affecting their interaction with T cells. Therefore, expression of IL-2R alpha (CD25) has been reported in lymphoid tissue (thymus, lymph nodes, spleen), peripheral blood, and localized sites of inflammation. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms |
| Involvement in Disease | Abnormal IL-2/IL-2R signaling can impair regulatory T cell (Treg) function, contributing to autoimmune diseases (Type 1 diabetes, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus). Aberrant expression of IL-2R alpha is noted in certain hematological malignancies, such as adult T cell leukemia/lymphoma and Hodgkin lymphoma. |
| UniProt | P01589 |
| Gene Symbol | IL2RA |
| Entrez Gene ID | 3559 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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