Recombinant Human PDGF-BB Protein
$90.00 – $4,199.00
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Key Features
✓ Endotoxin Level: <0.2 EU/1ug
✓ Purity: >96% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli
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Product Details
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Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL |
| Purity | >96% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Endotoxin | <0.2 EU per 1 µg of the protein as determined by the LAL method |
| Expression System | E. coli. Disulfide-linked homodimer |
| Accession Number | P01127.1 |
| Sequence | Ser82-Thr190
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT |
| Molecular Weight | 12.3 kDa (monomer, predicted), 13 kDa and 28 kDa (observed on SDS-PAGE gel stained with Coomassie® Blue under reducing and non-reducing conditions, respectively). |
| Formulation | Lyophilized from sterile Sodium Acetate and NaCl with Trehalose, without carrier protein. |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile 4 mM HCl. |
Data Images
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| Determined by a cell proliferation assay using mouse Balb/c 3T3 cells. The ED50 for this effect is typically 1-3 ng/mL | Recombinant Human PDGF-BB (2 ug/lane) on SDS-AGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing single bands at 13 kDa and 28 kDa, respectively, and purity greater than 96%. |
Background
| Alternative Names | Platelet Derived Growth Factor BB, PDGFBB, Platelet-Derived Growth Factor B, PDGFB, PDGF Subunit B, Epididymis Secretory Sperm Binding Protein, Oncogene SIS, SIS, Proto-Oncogene C-SIS, C-SIS, Becaplermin |
| Function | Platelet-Derived Growth Factor BB (PDGF-BB) is a protein with roles in regulating of many processes including embryonic development, angiogenesis, cell proliferation and differentiation. PDGF-BB acts on mesenchymal cells as a mitogen for connective tissue formation, particularly fibroblasts, vascular smooth muscle cells and glial cells. PDGF-BB is present in the alpha granules of platelets. As such, PDGF-BB promotes angiogenesis, chemotaxis of fibroblasts and collagen synthesis in tissue repair. Additionally, PDGF-BB supports development of kidney glomeruli, the differentiation of neural stem cells and has neuroprotective functions on mature neurons during injury. PDGF-BB primarily binds to the PDGFR beta (CD140B) homodimer, and with lower affinity to the PDGFR alpha (CD140a) homodimer or PDGFR alpha/PDGFR beta heterodimer leading to activation of the MAPK, PI3K, and STAT3 pathways. |
| Tissue Specificity | PDGF-BB expression has been identified in endothelial tissue, smooth muscle tissue, heart, lung, brain, and neurons. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of PDGF-BB is associated with atherosclerosis, pulmonary hypertension, glomerulonephritis, diabetic retinopathy, keloids, hypertrophic scars, cancer (glioma, sarcoma, and tumor angiogenesis). |
| UniProt | P01127 |
| Gene Symbol | PDGFB |
| Entrez Gene ID | 5155 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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