Recombinant Human R-Spondin 3/RSPO3 Protein
$90.00 – $3,199.00
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Key Features
✓ Endotoxin Level: Determined by LAL method
✓ Purity: Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
✓ Biological Activity: Yes
✓ Expression System: Mammalian cell line
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Product Details
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Product Documents
Product Details
| Biological Activity | Determined by in-house activity assay |
| Purity | Determined by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining |
| Endotoxin | Determined by LAL method |
| Expression System | Mammalian cell line. |
| Accession Number | Q9BXY4 |
| Sequence | Asn23-His272 NASRGRRQRR MHPNVSQGCQ GGCATCSDYN GCLSCKPRLF FALERIGMKQ IGVCLSSCPS GYYGTRYPDI NKCTKCKADC DTCFNKNFCT KCKSGFYLHL GKCLDNCPEG LEANNHTMEC VSIVHCEVSE WNPWSPCTKK GKTCGFKRGT ETRVREIIQH PSAKGNLCPP TNETRKCT VQRKKCQKGE RGKKGRERKR KKPNKGESKE AIPDSKSLES SKEIPEQREN KQQQKKRKVQ DKQKSVSVST VH |
| Molecular Weight | 28.2 kDa (predicted) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
Background
| Alternative Names | Roof Plate-specific Spondin 3, RSPO3, Thrombospondin Type I Domain Containing 2, Protein With TSP Type-1 Repeat, THSD2, PWTSR, CRISTIN1, HPWTSR, HRspo3, FLJ14440 |
| Function | Roof plate-specific Spondin 3 (R-Spondin 3 or RSPO3) is a key regulator of Wnt signaling with diverse functions in during embryonic development, tissue homeostasis, and stem cell maintenance. In embryonic development, RSPO3 is involved in various processes, including limb bud development, skeletal patterning, and organogenesis. In adults, it plays a role in maintaining tissue homeostasis, particularly in stem cell niches where Wnt signaling is critical for the regulation of stem cell self-renewal and differentiation. RSPO3 enhances Wnt signaling by competing with Wnt antagonist DKK-1 for binding to Low-Density Lipoprotein Receptor-related Proteins LRP4, LRP5 or LRP6 and inhibiting its interaction with the co-receptor Kremen. This inhibition leads to the stabilization of the Frizzled/LRP receptor complex, which initiates downstream signaling events mediated by Wnt proteins. As the in vivo roles of RSPO3 have been elucidated in tissue patterning and stem cell maintenance, RSPO3 plays a more common role for in vitro cell culture and the growth and maintenance of 3-D organoid cultures. |
| Tissue Specificity | R-Spondin 3 expression has been identified in limb buds, neural tube, Intestinal crypt epithelial cells, mammary glands, lung epithelium, liver bile ducts, basal layer of epidermis, hair follicles, ovaries and testes. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of R-Spondin 3 is associated with autosomal dominant polycystic kidney disease (ADPKD), brachydactyly type B1 (BDB1), Tetra-amelia syndrome, aplastic anemia and thrombocytopenia (TAAD), osteoarthritic cartilage, and numerous cancers (colorectal, gastric, breast, ovarian, liver and prostate). |
| UniProt | Q9BXY4 |
| Gene Symbol | RSPO3 |
| Entrez Gene ID | 84870 |
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