Recombinant Mouse IL-3 Protein
$90.00 – $3,199.00
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Key Features
✓ Endotoxin Level: <0.5 EU/1ug
✓ Purity: >95% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli
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Product Details
Storage & Preparation
Data Images
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Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using mouse NFS-60 cells. The ED50 for this effect is typically <0.1 ng/mL. |
| Purity | >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.5 EU per 1 ug of the protein as determined by the LAL method. |
| Expression System | E. coli |
| Accession Number | P01586 |
| Sequence | Asp33-Cys166, with an N-terminal Met
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC |
| Molecular Weight | 15.2 kDa (monomer, predicted) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cell proliferation assay using mouse NFS-60 cells. The ED50 for this effect is typically <0.1 ng/mL. | Recombinant Mouse IL-3 (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 15 kDa and purity greater than 95%. |
Background
| Alternative Names | Interleukin 3, IL3, Mast Cell Growth Factor, Multilineage Colony Stimulating Factor, Multipotential Colony Stimulating Factor, Hematopoietic Growth Factor, P-Cell Stimulating Factor, MULTI-CSF, MCGF, MGC79398, MGC79399 |
| Function | Interleukin-3 (IL-3) is a pleiotropic cytokine that plays a crucial role in the proliferation, differentiation, and survival of hematopoietic stem cells and myeloid progenitor cells. In particular, IL-3 regulates the development of basophils, dendritic cells, eosinophils, erythrocytes, macrophages, mast cells, megakaryocytes, monocytes, and neutrophils. IL-3 is produced by a variety of cells, including activated T cells, B cells, mast cells, and vascular endothelial cells. Signaling takes place through binding to IL-3R alpha (CD123) that heterodimerizes with the common beta chain (CD131). |
| Tissue Specificity | IL-3 expression has been identified in bone marrow stromal cells and vascular endothelial tissue. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of IL-3 is associated with asthma, allergic rhinitis, atopic dermatitis, acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), aplastic anemia, multiple sclerosis (MS), rheumatoid arthritis, systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), psoriasis, rheumatoid arthritis, and myelodysplastic syndromes (MDS). |
| UniProt | P01586 |
| Gene Symbol | IL3 |
| Entrez Gene ID | 16187 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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