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Key Features
✓ Detects endogenous beta-Actin
✓ Western blot, Immunohistochemistry, Immunofluorescence
✓ Monoclonal Mouse
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
Source | Monoclonal Mouse |
Specificity | Detects endogenous human, mouse, rat, bovine, canine, chicken, feline, fish, porcine, and sheep beta-Actin. |
Immunogen | Beta-Actin synthetic peptide, N-terminus (amino acids 1-80) |
Purification | Antigen affinity chromatography |
Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
Concentration | 1 mg/mL |
Applications | Western blot: 1:2,000-10,000 recommended dilution Immunofluorescence: 1:50-200 recommended dilution Immunohistochemistry: 1:100-300 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
Shipping | Shipped on blue ice. |
Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
Detection of beta-Actin by Western blot. Endogenous beta-Actin was detected at approximately 42 kDa in development maps of proteins from different sources: 1) control, 2) model, 3) Memantine, 4) FQD low dose, and 5) FQD high dose. |
Background
Alternative Names | b-Actin, ACTB |
Tissue Specificity | B-cell lymphoma, Brain, Cajal-Retzius cell, Eye, Fetal brain cortex, Hepatocellular carcinoma |
Cellular Localization | Cytoplasm, cytoskeleton, Nucleus |
UniProt | Human: P60709 Mouse: P60710 Rat: P60711 |
Gene Symbol | ACTB |
Entrez Gene ID | Human: 60 Mouse: 11461 Rat: 81822 |
Product Documents
Product Datasheet | Download Product Datasheet |
COA | Contact Us |
SDS | Contact Us |
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