DYKDDDDK Tag Antibody
$175.00
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Key Features
✓Detects both N-terminal and C-terminal DYKDDDDK (FLAG®) epitope-tagged proteins.
✓ Western blot, Immunofluorescence, Immunoprecipitation
✓ Recombinant Monoclonal Mouse IgG1
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Source | Recombinant Monoclonal Mouse IgG1 |
| Specificity | Detects both N-terminal and C-terminal DYKDDDDK (FLAG®) epitope-tagged proteins. |
| Immunogen | DYKDDDDK synthetic peptide |
| Purification | Antigen affinity chromatography |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
| Concentration | 1 mg/mL |
| Applications | Western blot: 1:10,000-20,000 recommended dilution Immunofluorescence: 1:1,000-3,000 recommended dilution Immunoprecipitation: 1:100-200 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
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| Detection of DYKDDDDK Tag by Western blot. DYKDDDDK epitope-tagged proteins were detected using Catalog # EXAB006 at 1:10,000 dilution, secondary antibody at 1:10,000 dilution. Non-tagged proteins were loaded in lanes 1, 3, 5 and DYKDDDDK tagged proteins were loaded in lanes 2, 4, and 6. |
Background
| Alternative Names | Flag tag, Flag-tag, DDDDK TAG, DDDDK-TAG, DYKDDDDK tag, DYKDDDDK-tag |
FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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