GAPDH Antibody
$175.00
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Key Features
✓ Detects endogenous GAPDH
✓ Western blot, Immunohistochemistry, Immunofluorescence
✓ Monoclonal Mouse
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Source | Monoclonal Mouse |
| Specificity | Detects human, mouse, rat, canine, chicken, hamster, porcine, rabbit, sheep, insect, and yeast GAPDH. |
| Immunogen | GAPDH synthetic peptide |
| Purification | Antigen affinity chromatography |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
| Concentration | 1 mg/mL |
| Applications | Western blot: 1:5,000-20,000 recommended dilution Immunofluorescence: 1:200 recommended dilution Immunohistochemistry: 1:200-300 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
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| Detection of GAPDH by Western blot. Endogenous GAPDH was detected at approximately 37 kDa using Catalog # EXAB005 at 1:10,000 dilution, secondary antibody at 1:10,000 dilution. Samples were loaded into the lanes: 1) HeLa cells, 2) rat brain tissue, 3) rabbit muscle tissue, 4) sheep muscle tissue, and 5) mouse brain tissue. |
Background
| Alternative Names | Glyceraldehyde-3-phosphate dehydrogenase, GAPD, CDABP0047, OK/SW-cl.12 |
| Tissue Specificity | Astrocytoma, Brain, Cajal-Retzius cell, Colon adenocarcinoma, Epithelial tissue |
| Cellular Localization | Cytoplasm, cytosol, nucleus, cytoplasm, perinuclear region, cell membrane |
| UniProt | Human: P04406 Mouse: P16858 Rat: P04797 |
| Gene Symbol | GAPDH |
| Entrez Gene ID | Human: 2597 Mouse: 100042025 Rat: 24383 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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