His Tag Antibody
$199.00
Product will arrive in 1 to 2 business days
Key Features
✓ Detects poly-histidine-tagged fusion proteins
✓ Western blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
✓ Monoclonal Mouse
Size
✓ 100 ug
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Source | Monoclonal Mouse |
| Specificity | Detects C-terminal, internal, and N-terminal poly-histidine-tagged fusion proteins. |
| Immunogen | KLH-conjugated His-tag synthetic peptide |
| Purification | Antigen affinity chromatography |
| Formulation | PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as a preservative and 50% glycerol. |
| Concentration | 1 mg/mL |
| Applications | Western blot: 1:3000 recommended dilution Immunofluorescence: 1:1000 recommended dilution Immunohistochemistry: 1:1000 recommended dilution Immunoprecipitation: 1:2000 recommended dilution For optimal performance, this reagent should be titrated for each application. |
Storage & Preparation
| Shipping | Shipped on blue ice. |
| Stability & Storage | 12 months from date of receipt at -15°C to -20°C. |
Data Images
Detection of His Tag by Western blot. This antibody detects poly-His-tagged fusion proteins at 1:5000 dilution (lane 1) and 1:10000 dilution (lane 2).
Background
| Background | The poly-histidine tag (commonly referred to as His-tag) consists of multiple consecutive histidine residues inserted into the amino acid sequence of recombinant proteins. The His-tag facilitates detection by antibodies, binding for purification and downstream applications. |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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