Recombinant Human EGF Protein, Animal Component-Free
$90.00 – $259.00
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Key Features
✓ Endotoxin Level: <0.01 EU/1ug
✓ Purity: >97% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli using an animal component-free system
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Product Details
Storage & Preparation
Data Images
Background
Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using Balb/c-3T3 mouse fibroblast cells. The ED50 for this effect is typically <100 pg/mL. |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.01 EU/1ug of the protein as determined by the LAL method. |
| Expression System | E. coli using an animal component-free system. |
| Accession Number | P01133 |
| Sequence | Asn971-Arg1023, with an N-terminal Met
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR |
| Molecular Weight | 6.4 kDa (predicted) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cell proliferation assay using Balb/c-3T3 mouse fibroblast cells. The ED50 for this effect is typically <100 pg/mL. | Recombinant Human EGF (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 6 kDa and purity greater than 97%. |
Background
| Alternative Names | Epidermal Growth Factor, Beta-Urogastrone, Urogastrone, URG, HOMG4 |
| Function | EGF (Epidermal Growth Factor) is a growth factor that plays several roles related to cell growth, proliferation, and differentiation mediated through binding to its receptor, EGFR (Epidermal Growth Factor Receptor). EGF may be expressed in macrophages, platelets, plasma, milk, urine, saliva, seminal fluid, amniotic fluid and cerebrospinal fluid. Several roles are attributed to EGF such as angiogenesis, epithelial cell growth and maintenance, keratinocyte proliferation, gastrointestinal mucosa integrity, along with neurogenesis and neuronal survival. Due to these wide-ranging functions as a growth factor, EGF is commonly used in cell culture for the maintenance and growth of cell lines, stem cells and 3-D organoids. |
| Tissue Specificity | EGF expression has been reported in skin, gastrointestinal epithelial tissue, salivary glands, kidney, brain, liver, mammary glands, ovaries and testes. |
| Cellular Localization | Membrane-bound and cleaved soluble protein forms. |
| Involvement in Disease | Dysregulation of EGF is associated with several cancers (lung cancer, breast cancer, colorectal cancer, and head and neck cancer), psoriasis, inflammatory bowel disease (IBD), diabetic nephropathy, polycystic kidney disease, peptic ulcers and impaired wound healing. |
| UniProt | P01133 |
| Gene Symbol | EGF |
| Entrez Gene ID | 1950 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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