Recombinant Human G-CSF Protein
$90.00 – $4,499.00
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Key Features
✓ Endotoxin Level: <0.02 EU/1ug
✓ Purity: >97% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: E. coli
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Product Details
Storage & Preparation
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Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL. |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.02 EU/1ug of the protein as determined by the LAL method. |
| Expression System | E. coli |
| Accession Number | NP_757373.1 |
| Sequence | Thr31-Pro204
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP |
| Molecular Weight | 18.7 kDa (monomer, predicted) |
| Formulation | Lyophilized from sterile Sodium Acetate and Sodium Chloride with Trehalose. |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL. | Recombinant Human G-CSF (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at approximately 17 kDa and purity greater than 97%. |
Background
| Alternative Names | Granulocyte Colony Stimulating Factor, Colony Stimulating Factor 3GCSF, CSF3, C17orf33, CSF3OS, Filgrastim, Pluripoietin, Lenograstim |
| Function | G-CSF primarily stimulates the proliferation, differentiation, and survival of neutrophilic granulocyte precursors in the bone marrow and stimulates upregulation of mature neutrophil release into the bloodstream as part of the innate immune response. During an immune response, G-CSF is secreted by monocytes, macrophages, and activated T cells. G-CSF binds to the G-CSF receptor (G-CSFR), which is expressed on the surface of hematopoietic cells, particularly myeloid progenitors. G-CSF signaling occurs via the JAK/STAT, Ras/MAPK and PI3K/Akt pathways. |
| Tissue Specificity | G-CSF expression has been reported in bone marrow stromal cells, fibroblasts, endothelial cells, and epithelial cells. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of G-CSF is implicated in leukemias (such as acute myeloid leukemia) and myelodysplastic syndromes, autoimmune disorders (rheumatoid arthritis and inflammatory bowel disease), congenital neutropenia, and solid tumors where aberrant expression may contribute to tumor progression, metastasis, and the formation of a supportive tumor microenvironment. |
| UniProt | P09919-2 |
| Gene Symbol | CSF3 |
| Entrez Gene ID | 1440 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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