Recombinant Human IL-6 Protein
$90.00 – $3,199.00
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Key Features
✓ Endotoxin Level: <1.0 EU/1ug
✓ Purity: >97% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: CHO cell line
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Product Details
Storage & Preparation
Data Images
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Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using DS1 mouse lymphoblast cells. The ED50 for this effect is typically 10-100 pg/mL. |
| Purity | >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <1.0 EU/1ug of the protein as determined by the LAL method. |
| Expression System | CHO cell line. |
| Accession Number | P05231 |
| Sequence | Val30-Met212
VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM |
| Molecular Weight | 20.8 kDa (predicted), 20-23 kDa (glycosylated, observed on SDS-PAGE gel stained with Coomassie® Blue) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cell proliferation assay using DS1 mouse lymphoblast cells. The ED50 for this effect is typically 10-100 pg/mL. | Recombinant Human IL-6 (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue with the observed glycosylated molecular weight at 20-23 kDa and purity greater than 97%. |
Background
| Alternative Names | Interleukin 6, Interferon Beta-2, IFN-Beta-2, IFNB2, B-Cell Stimulatory Factor 2, BSF2, Hybridoma Growth Factor, HGF, HSF, CTL Differentiation Factor, CDF |
| Function | IL-6 plays a role in regulating immune response, inflammation, hematopoiesis, metabolism, and neurological processes. IL-6 influences the growth and differentiation of hematopoietic progenitor and stem cells. In the innate immune response, IL-6 is released by macrophages and dendritic cells. During the adaptive immune response, IL-6 plays a role in B cell maturation and differentiation of T cells into T follicular helper (Tfh) cells and Th17 cells. Muscle tissue and the central nervous system response to IL-6 has been linked to lipolysis and modulation of insulin response. IL-6 signaling occurs via binding to IL-6R alpha (CD126) and subsequent dimerization with the signal-transducing gp130 subunit. |
| Tissue Specificity | IL-6 expression has been reported in muscle tissue, endothelial tissue, lung, intestine, and adipose tissue. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of IL-6 is implicated in rheumatoid arthritis, systemic juvenile idiopathic arthritis (sJIA) inflammatory bowel disease (IBD), autoimmune hepatitis, atherosclerosis, insulin resistance, type 2 diabetes, fatty liver disease, obesity-related inflammation, Castleman disease, Behçet’s disease Alzheimer’s disease, systemic lupus erythematosus, multiple myeloma, and various cancers (prostate, breast, and lung) through actions on the tumor microenvironment. |
| UniProt | P05231 |
| Gene Symbol | IL6 |
| Entrez Gene ID | 3569 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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