Recombinant Human Thrombopoietin/TPO Protein
$90.00 – $4,599.00
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Key Features
✓ Endotoxin Level: <0.1 EU/1ug
✓ Purity: >95% SDS-PAGE
✓ Biological Activity: Yes
✓ Expression System: CHO cell line
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Product Details
Storage & Preparation
Data Images
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Product Documents
Product Details
| Biological Activity | Determined by a cell proliferation assay using MO7e human cells. The ED50 for this effect is typically <10 ng/mL. |
| Purity | >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining. |
| Endotoxin | <0.1 EU/1ug of the protein as determined by the LAL method. |
| Expression System | CHO cell line. |
| Accession Number | P40225 |
| Sequence | Ser22-Leu195
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL |
| Molecular Weight | 18.7 kDa (monomer, predicted), 20 kDa and 18 kDa (glycosylated, observed on SDS-PAGE gel stained using Coomassie® Blue under reducing and non-reducing conditions, respectively) |
| Formulation | Lyophilized from sterile PBS with Trehalose, pH 7.4 |
Storage & Preparation
| Shipping | Shipped at ambient temperature. |
| Stability & Storage |
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| Reconstitution | Reconstitute at 100 ug/mL in sterile PBS. |
Data Images
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| Determined by a cell proliferation assay using MO7e human cells. The ED50 for this effect is typically <10 ng/mL. | Recombinant Human Thrombopoietin/ TPO Protein (2 ug/lane) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained using Coomassie® Blue showing a single band at 20 kDa and 18 kDa, respectively, and purity greater than 95%. |
Background
| Alternative Names | Thrombopoietin, THPO, TPO, Myeloproliferative Leukemia Virus Oncogene Ligand, Megakaryocyte Growth And Development Factor, Megakaryocyte Colony-Stimulating Factor, Thrombopoietin Nirs, C-Mpl Ligand, MPLLG, MGDF, THCYT1, CAMT2, MKCSF |
| Function | Thrombopoietin (TPO) plays an important role in the proliferation and maturation of megakaryocytes and their progenitors in regulating platelet production. TPO has a role in promoting the survival, self-renewal, and expansion of hematopoietic stem cells, along with primitive multilineage progenitor cells. This has led to further interest in applications for in vitro and ex vivo maintenance, expansion and differentiation of hematopoietic stem cells. TPO is the ligand for c-Mpl (CD110). |
| Tissue Specificity | TPO is primarily synthesized in the liver, and to lesser degrees in the kidneys, bone marrow and spleen. |
| Cellular Localization | Secreted protein |
| Involvement in Disease | Dysregulation of TPO is associated with idiopathic thrombocytopenic purpura (ITP), aplastic anemia, myelodysplastic syndromes (MDS), thrombocytosis, hepatitis, cirrhosis, and chemotherapy-induced thrombocytopenia. |
| UniProt | P40225 |
| Gene Symbol | THPO |
| Entrez Gene ID | 7066 |
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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